Book , Print in English

Advanced genetic analysis : finding meaning in a genome

R. Scott Hawley and Michelle Y. Walker.
  • Malden, Mass. : Blackwell Pub., ©2003.
  • xv, 239 pages : illustrations ; 25 cm
Subjects
Medical Subjects
Local Subjects
Summary
  • Advanced Genetic Analysis brings a state-of-the-art, exciting new approach to genetic analysis. Focusing on the underlying principles of modern genetic analysis, this book provides the 'how' and 'why' of the essential analytical tools needed. The author's vibrant, accessible style provides an easy guide to difficult genetic concepts, from mutation and gene function to gene mapping and chromosome segregation. Throughout, a balanced range of model organisms and timely examples are used to illustrate the theoretical basics.
Contents
  • note: Ch. 1 Mutation
  • 1.1. Types of mutations
  • 1.2. Dominance and recessivity
  • 1.3. genetic utility of dominant and recessive mutants
  • Ch. 2 Mutant hunts
  • 2.1. Why look for new mutants?
  • 2.2. Mutagenesis and mutational mechanisms
  • 2.3. What phenotype should you screen (or select) for?
  • 2.4. Actually getting started
  • Ch. 3 complementation test
  • 3.1. essence of the complementation test
  • 3.2. Rules for using the complementation test
  • 3.3. How might the complementation test lie to you?
  • 3.4. Second-site non-complementation (SSNC) (non-allelic non-complementation)
  • 3.5. extension of second-site non-complementation: dominant enhancers
  • Ch. 4 Suppression
  • 4.1. basic definition of genetic suppression
  • 4.2. Intragenic suppression (pseudo-reversion)
  • 4.3. Extragenic suppression
  • 4.4. Transcriptional suppression
  • 4.5. Translational suppression
  • 4.6. Suppression by post-translational modification
  • 4.7. Extragenic suppression as a result of protein-protein interaction
  • 4.8. Suppression without physical interaction
  • 4.9. Suppression of dominant mutations
  • 4.10. Designing your own screen for suppressor mutations
  • Ch. 5 Determining when and where genes function
  • 5.1. Epistasis: ordering gene function in pathways
  • 5.2. Mosaic analysis: where does a given gene act?
  • Ch. 6 Genetic fine-structure analysis
  • 6.1. Intragenic mapping (then)
  • 6.2. Intragenic mapping (now)
  • 6.3. Intragenic complementation meets intragenic recombination: the basis of fine-structure analysis
  • 6.4. example of fine-structure analysis for a eukaryotic gene encoding a multifunctional protein
  • 6.5. Fine-structure analysis of genes with complex regulatory elements in eukaryotes
  • 6.6. Pairing-dependent intragenic complementation
  • Ch. 7 Meiotic recombination
  • 7.1. introduction to meiosis
  • 7.2. Crossingover and chiasmata: recombination involves the physical interchange of genetic material and ensures homolog separation
  • 7.3. classical analysis of recombination
  • 7.4. Measuring the frequency of recombination
  • 7.5. mechanism of recombination
  • Ch. 8 Meiotic chromosome segregation
  • 8.1. Types and consequences of failed segregation
  • 8.2. origin of spontaneous nondisjunction
  • 8.3. centromere
  • 8.4. Segregational mechanisms.
Other information
  • Includes bibliographical references (pages 220-235) and indexes.
  • OCLC
ISBN
  • 1405103361
  • 9781405103367
Identifying numbers
  • LCCN: 2002071233
  • OCLC: 49795681
  • OCLC: 49795681